Biocatalysts and Enzyme Technology

Author: Klaus Buchholz,Volker Kasche,Uwe Theo Bornscheuer

Publisher: John Wiley & Sons

ISBN: 3527672001

Category: Science

Page: 626

View: 5449

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This second edition of a bestselling textbook offers an instructive and comprehensive overview of our current knowledge of biocatalysis and enzyme technology. The book now contains about 40% more printed content. Three chapters are completely new, while the others have been thoroughly updated, and a section with problems and solutions as well as new case studies have been added. Following an introduction to the history of enzyme applications, the text goes on to cover in depth enzyme mechanisms and kinetics, production, recovery, characterization and design by protein engineering. The authors treat a broad range of applications of soluble and immobilized biocatalysts, including wholecell systems, the use of non-aqueous reaction systems, applications in organic synthesis, bioreactor design and reaction engineering. Methods to estimate the sustainability, important internet resources and their evaluation, and legislation concerning the use of biocatalysts are also covered.

Enzyme Technologies

Metagenomics, Evolution, Biocatalysis and Biosynthesis

Author: Wu-Kuang Yeh,Hsiu-Chiung Yang,James R. McCarthy

Publisher: John Wiley and Sons

ISBN: 1118125037

Category: Science

Page: 368

View: 823

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Biotechnology of Microbial Enzymes

Production, Biocatalysis and Industrial Applications

Author: Goutam Brahmachari

Publisher: Academic Press

ISBN: 0128037466

Category: Medical

Page: 632

View: 8703

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Biotechnology of Microbial Enzymes: Production, Biocatalysis and Industrial Applications provides a complete survey of the latest innovations on microbial enzymes, highlighting biotechnological advances in their production and purification along with information on successful applications as biocatalysts in several chemical and industrial processes under mild and green conditions. Applications of microbial enzymes in food, feed, and pharmaceutical industries are given particular emphasis. The application of recombinant DNA technology within industrial fermentation and the production of enzymes over the last 20 years have produced a host of useful chemical and biochemical substances. The power of these technologies results in novel transformations, better enzymes, a wide variety of applications, and the unprecedented development of biocatalysts through the ongoing integration of molecular biology methodology, all of which is covered insightfully and in-depth within the book. Features research on microbial enzymes from basic science through application in multiple industry sectors for a comprehensive approach Includes information on metabolic pathway engineering, metagenomic screening, microbial genomes, extremophiles, rational design, directed evolution, and more Provides a holistic approach to the research of microbial enzymes

Practical Methods for Biocatalysis and Biotransformations 3

Author: John Whittall,Peter W. Sutton,Wolfgang Kroutil

Publisher: John Wiley & Sons

ISBN: 1118696298

Category: Technology & Engineering

Page: 320

View: 7102

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Biocatalysts are increasingly used by chemists engaged in fine chemical synthesis within both industry and academia. Today, there exists a huge choice of high-tech enzymes and whole cell biocatalysts, which add enormously to the repertoire of synthetic possibilities. Practical Methods for Biocatalysis and Biotransformations 3 will be a companion book to Practical Methods for Biocatalysis and Biotransformations (2009) and Practical Methods for Biocatalysis and Biotransformations 2 (2012). Following the successful format of the two volumes, it will be a “how-to” guide focusing on commercially available enzymes and strains of microorganisms that are readily obtained from culture collections. The source of starting materials and reagents, hints, tips and safety advice (where appropriate) will be given to ensure, as far as possible, that the procedures are reproducible. Comparisons to alternative methodology will be given and relevant references to the primary literature will be cited. Contents include: Biotransformation Process Technology Industrial Biooxidation Hydrolase catalysed hydrolysis/synthesis Reduction Oxidation Halogenation Transferase catalysed glycosylation, methylation, etc C-C bond formation Tandem Biocatalytic Reactions Practical Methods for Biocatalysis and Biotransformations, Volume 3 is an essential collection of validated biocatalytic methods which will find a place on the bookshelves of synthetic organic chemists, pharmaceutical chemists, and process R&D chemists in industry and academia.

Future Directions in Biocatalysis

Author: Tomoko Matsuda

Publisher: Elsevier

ISBN: 0444637508

Category: Technology & Engineering

Page: 460

View: 5173

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Future Directions in Biocatalysis, Second Edition, presents the future direction and latest research on how to utilize enzymes, i.e., natural catalysts, to make medicines and other necessities for humans. It emphasizes the most important and unique research on biocatalysis instead of simply detailing the ABC’s on the topic. This book is an indispensable tool for new researchers in the field to help identify specific needs, start new projects that address current environmental concerns, and develop techniques based on green technology. It provides invaluable hints and clues for conducting new research on enzymes, with final sections outlining future directions in biocatalysis further expanding the science into new applications. Gives future directions in the area of biocatalysis research Presents research topics based on their uniqueness, originality, and novelty Includes many explanatory figures to demonstrate concepts to both organic chemists and biochemists Shows that there is no boundary between organic chemistry and biochemistry

Enzyme Biocatalysis

Principles and Applications

Author: Andrés Illanes

Publisher: Springer Science & Business Media

ISBN: 1402083610

Category: Technology & Engineering

Page: 392

View: 8949

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This book was written with the purpose of providing a sound basis for the design of enzymatic reactions based on kinetic principles, but also to give an updated vision of the potentials and limitations of biocatalysis, especially with respect to recent app- cations in processes of organic synthesis. The ?rst ?ve chapters are structured in the form of a textbook, going from the basic principles of enzyme structure and fu- tion to reactor design for homogeneous systems with soluble enzymes and hete- geneous systems with immobilized enzymes. The last chapter of the book is divided into six sections that represent illustrative case studies of biocatalytic processes of industrial relevance or potential, written by experts in the respective ?elds. We sincerely hope that this book will represent an element in the toolbox of gr- uate students in applied biology and chemical and biochemical engineering and also of undergraduate students with formal training in organic chemistry, biochemistry, thermodynamics and chemical reaction kinetics. Beyond that, the book pretends also to illustrate the potential of biocatalytic processes with case studies in the ?eld of organic synthesis, which we hope will be of interest for the academia and prof- sionals involved in R&D&I. If some of our young readers are encouraged to engage or persevere in their work in biocatalysis this will certainly be our more precious reward.

Enzymes in Industry

Author: Wolfgang Aehle

Publisher: John Wiley & Sons

ISBN: 3527617108

Category: Science

Page: 516

View: 1176

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Leading experts from all over the world present an overview of the use of enzymes in industry for: - the production of bulk products, such as glucose, or fructose - food processing and food analysis - laundry and automatic dishwashing detergents - the textile, pulp and paper and animal feed industries - clinical diagnosis and therapy - genetic engineering. The book also covers identification methods of new enzymes and the optimization of known ones, as well as the regulatory aspects for their use in industrial applications. Up to date and wide in scope, this is a chance for non-specialists to acquaint themselves with this rapidly growing field. '...The quality...is so great that there is no hesitation in recommending it as ideal reading for any student requiring an introduction to enzymes. ...Enzymes in Industry - should command a place in any library, industrial or academic, where it will be frequently used.' The Genetic Engineer and Biotechnologist 'Enzymes in Industry' is an excellent introduction into the field of applied enzymology for the reader who is not familiar with the subject. ... offers a broad overview of the use of enzymes in industrial applications. It is up-to-date and remarkable easy to read, despite the fact that almost 50 different authors contributed. The scientist involved in enzyme work should have this book in his or her library. But it will also be of great value to the marketing expert interested in the present use of enzymes and their future in food and nonfood applications.' Angewandte Chemie 'This book should be available to all of those working with, or aspiring to work with, enzymes. In particular academics should use this volume as a source book to ensure that their 'new' projects will not 'reinvent the wheel'.' Journal of Chemical Technology and Biotechnology

Biocatalysis and Nanotechnology

Author: Peter Grunwald

Publisher: CRC Press

ISBN: 1351767542

Category: Medical

Page: 836

View: 3965

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This book provides an actual overview of the various relations between nanotechnology and biocatalysis. Recently, nanobiocatalysis has rapidly developed to a subarea of enzyme biotechnology. It combines the advances in nanotechnology that have generated nanoscale materials of different sizes, shapes, and physicochemical properties, with the excellent characteristics of biocatalysts to an innovative technology. The book discusses the fabrication and application of these nanomaterials for the immobilization of enzymes for the sustainable production of goods and chemicals. Nanosupports are of advantage compared with bulk solid materials due to their high surface area resulting in a comparatively high enzyme loading and a significantly reduced mass transfer limitation. These characteristics are also of great use for applications in the fields of enzymatic biosensors, biofuel cells, bioelectronics, and photoelectrochemical analyte detection, where conductive nanomaterials improve the rate of electron transfer. Furthermore, nanostructured enzyme catalysis in organic solvents and its potential application for biodiesel production, probing of enzymatic activity, and identification of enzyme functions or inorganic nanoparticles as enzyme mimics together with an overview of nanotoxicology are also covered in this book.

Novel Enzyme Technology for Food Applications

Author: R Rastall

Publisher: Elsevier

ISBN: 184569371X

Category: Technology & Engineering

Page: 336

View: 1408

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The food industry is constantly seeking advanced technologies to meet consumer demand for nutritionally balanced food products. Enzymes are a useful biotechnological processing tool whose action can be controlled in the food matrix to produce higher quality products. Written by an international team of contributors, Novel enzyme technology for food applications reviews the latest advanced methods to develop specific enzymes and their applications. Part one discusses fundamental aspects of industrial enzyme technology. Chapters cover the discovery, improvement and production of enzymes as well as consumer attitudes towards the technology. Chapters in Part two discuss enzyme technology for specific food applications such as textural improvement, protein-based fat replacers, flavour enhancers, and health-functional carbohydrates. Novel enzyme technology for food applications is a standard reference for all those in industry and academia concerned with improving food products with this advanced technology. Reviews the latest advanced methods to develop specific enzymes Discusses ways of producing higher quality food products Explores the improvement and production of enzymes

Applied Biocatalysis

From Fundamental Science to Industrial Applications

Author: Lutz Hilterhaus,Andreas Liese,Ulrich Kettling,Garabed Antranikian

Publisher: John Wiley & Sons

ISBN: 3527677143

Category: Science

Page: 400

View: 2586

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This reference book originates from the interdisciplinary research cooperation between academia and industry. In three distinct parts, latest results from basic research on stable enzymes are explained and brought into context with possible industrial applications. Downstream processing technology as well as biocatalytic and biotechnological production processes from global players display the enormous potential of biocatalysts. Application of "extreme" reaction conditions (i.e. unconventional, such as high temperature, pressure, and pH value) - biocatalysts are normally used within a well defined process window - leads to novel synthetic effects. Both novel enzyme systems and the synthetic routes in which they can be applied are made accessible to the reader. In addition, the complementary innovative process technology under unconventional conditions is highlighted by latest examples from biotech industry.

Biocatalysis for Green Chemistry and Chemical Process Development

Author: Junhua (Alex) Tao,Romas Joseph Kazlauskas

Publisher: John Wiley & Sons

ISBN: 9781118028292

Category: Technology & Engineering

Page: 480

View: 6814

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This book describes recent progress in enzyme-driven green syntheses of industrially important molecules. The first three introductory chapters overview recent technological advances in enzymes and cell-based transformations, and green chemistry metrics for synthetic efficiency. The remaining chapters are directed to case studies in biotechnological production of pharmaceuticals (small molecules, natural products and biologics), flavors, fragrance and cosmetics, fine chemicals, value-added chemicals from glucose and biomass, and polymeric materials. The book is aimed to facilitate the industrial applications of this powerful and emerging green technology, and catalyze the advancement of the technology itself.

Green Biocatalysis

Author: Ramesh N. Patel

Publisher: John Wiley & Sons

ISBN: 1118822358

Category: Technology & Engineering

Page: 792

View: 5164

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Green Biocatalysis presents an exciting green technology that uses mild and safe processes with high regioselectivity and enantioselectivity. Bioprocesses are carried out under ambient temperature and atmospheric pressure in aqueous conditions that do not require any protection and deprotection steps to shorten the synthetic process, offering waste prevention and using renewable resources. Drawing on the knowledge of over 70 internationally renowned experts in the field of biotechnology, Green Biocatalysis discusses a variety of case studies with emphases on process R&D and scale-up of enzymatic processes to catalyze different types of reactions. Random and directed evolution under process conditions to generate novel highly stable and active enzymes is described at length. This book features: A comprehensive review of green bioprocesses and application of enzymes in preparation of key compounds for pharmaceutical, fine chemical, agrochemical, cosmetic, flavor, and fragrance industries using diverse enzymatic reactions Discussion of the development of efficient and stable novel biocatalysts under process conditions by random and directed evolution and their applications for the development of environmentally friendly, efficient, economical, and sustainable green processes to get desired products in high yields and enantiopurity The most recent technological advances in enzymatic and microbial transformations and cuttingedge topics such as directed evolution by gene shuffling and enzyme engineering to improve biocatalysts With over 3000 references and 800 figures, tables, equations, and drawings, Green Biocatalysis is an excellent resource for biochemists, organic chemists, medicinal chemists, chemical engineers, microbiologists, pharmaceutical chemists, and undergraduate and graduate students in the aforementioned disciplines.

Biocatalysis for the Pharmaceutical Industry

Discovery, Development, and Manufacturing

Author: Junhua (Alex) Tao,Guo-Qiang Lin,Andreas Liese

Publisher: John Wiley & Sons

ISBN: 0470823151

Category: Science

Page: 250

View: 4731

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Biocatalysis is rapidly evolving into a key technology for the discovery and production of chemicals, especially in the pharmaceutical industry, where high yielding chemo-, regio-, and enantioselective reactions are critical. Taking the latest breakthroughs in genomics and proteomics into consideration, Biocatalysis for the Pharmaceutical Industry concisely yet comprehensively discusses the modern application of biocatalysis to drug discovery, development, and manufacturing. Written by a team of leading experts, the book offers deep insight into this cutting edge field. Covers a wide range of topics in a systematic manner with an emphasis on industrial applications Provides a thorough introduction to the latest biocatalysts, modern expression hosts, state-of-the-art directed evolution, high throughput screening, and bioprocess engineering Addresses frontier subjects such as emerging enzymes, metabolite profiling, combinatorial biosynthesis, metabolic engineering, and autonomous enzymes for the synthesis and development of chiral molecules, drug metabolites, and semi-synthetic medicinal compounds and natural product analogs Highlights the impact of biocatalysis on green chemistry Contains numerous graphics to illustrate concepts and techniques Biocatalysis for the Pharmaceutical Industry is an essential resource for scientists, engineers, and R&D policy makers in the fine chemical, pharmaceutical, and biotech industries. It is also an invaluable tool for academic researchers and advanced students of organic and materials synthesis, chemical biology, and medicinal chemistry.

Organic Synthesis Using Biocatalysis

Author: Animesh Goswami,Jon D. Stewart

Publisher: Academic Press

ISBN: 012411542X

Category: Science

Page: 438

View: 8082

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Organic Synthesis Using Biocatalysis provides a concise background on the application of biocatalysis for the synthesis of organic compounds, including the important biocatalytic reactions and application of biocatalysis for the synthesis of organic compounds in pharmaceutical and non-pharmaceutical areas. The book provides recipes for carrying out various biocatalytic reactions, helping both newcomers and non-experts use these methodologies. It is written by experts in their fields, and provides both a current status and future prospects of biocatalysis in the synthesis of organic molecules. Provides a concise background of the application of biocatalysis for the synthesis of organic compounds Expert contributors present recipes for carrying out biocatalytic reactions, including subject worthy discussions on biocatalysis in organic synthesis, biocatalysis for selective organic transformation, enzymes as catalysis for organic synthesis, biocatalysis in Industry, including pharmaceuticals, and more Contains detailed, separate chapters that describe the application of biocatalysis

Enzyme Technology

Author: Martin F. Chaplin,C. Bucke

Publisher: CUP Archive

ISBN: 9780521348843

Category: Science

Page: 264

View: 7074

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This textbook provides a clear and authoritative guide to the principles and practice of the utilization of enzymes in biotechnology. Enzymes have increasingly important applications in the food and pharmaceutical industry, in medicine, and as biosensors.

Problem Solving in Enzyme Biocatalysis

Author: Andrés Illanes,Lorena Wilson,Carlos Vera

Publisher: John Wiley & Sons

ISBN: 1118341775

Category: Technology & Engineering

Page: 344

View: 478

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Enzyme biocatalysis is a fast-growing area in process biotechnology that has expanded from the traditional fields of foods, detergents, and leather applications to more sophisticated uses in the pharmaceutical and fine-chemicals sectors and environmental management. Conventional applications of industrial enzymes are expected to grow, with major opportunities in the detergent and animal feed sectors, and new uses in biofuel production and human and animal therapy. In order to design more efficient enzyme reactors and evaluate performance properly, sound mathematical expressions must be developed which consider enzyme kinetics, material balances, and eventual mass transfer limitations. With a focus on problem solving, each chapter provides abridged coverage of the subject, followed by a number of solved problems illustrating resolution procedures and the main concepts underlying them, plus supplementary questions and answers. Based on more than 50 years of teaching experience, Problem Solving in Enzyme Biocatalysis is a unique reference for students of chemical and biochemical engineering, as well as biochemists and chemists dealing with bioprocesses. Contains: Enzyme properties and applications; enzyme kinetics; enzyme reactor design and operation 146 worked problems and solutions in enzyme biocatalysis.

Lipid Modification by Enzymes and Engineered Microbes

Author: Uwe T. Bornscheuer

Publisher: Elsevier

ISBN: 0128131683

Category: Science

Page: 394

View: 414

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Lipid Modification by Enzymes and Engineered Microbes covers the state-of-the art use of enzymes as natural biocatalysts to modify oils, also presenting how microorganisms, such as yeast, can be designed. In the past ten years, the field has made enormous progress, not only with respect to the tools developed for the development of designer enzymes, but also in the metabolic engineering of microbes, the discovery of novel enzyme activities, and in reaction engineering/process development. For the first time, these advances are covered in a single-volume that is edited by leading enzymatic scientist Uwe Borchscheuer and authored by an international team of experts. Identifies how, and when, to use enzymes and microbes for lipid modification Provides enzymatic, microbial and metabolic techniques for lipid modification Covers lipases, acyltransferases, phospholipases, lipoxygenases, monooxygenases, isomerases and sophorolipids Includes lipid modification for use in food, biofuels, oleochemicals and polymer precursors

Biocatalysis and Agricultural Biotechnology

Author: Ching T. Hou,Jei-Fu Shaw

Publisher: CRC Press

ISBN: 9781420077070

Category: Science

Page: 442

View: 2337

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Worldwide energy and food crises are spotlighting the importance of bio-based products – an area many are calling on for solutions to these shortages. Biocatalysis and Agricultural Biotechnology encapsulates the cutting-edge advances in the field with contributions from more than 50 international experts comprising sectors of academia, industry, and government research institutes, a virtual Who’s Who among biocatalysis scientists. Created Under the Editorial Guidance of Leading Biotechnology Experts With the aid of numerous graphs and illustrations, this authoritative reference documents such important advances as: Cloning and characterization of Kennedy pathway acyltransferases Engineering of plants for industrial uses New approaches from acquired tolerance to the biotic and abiotic stress of economically important crops This comprehensive text also explores a variety of bio-based industrial products, including: The modification of enzyme character through gene manipulation The biocatalytic synthesis of chiral intermediates for drug development The use of Omega-3 phospholipid nano capsules as effective forms for transporting immune response modifiers Providing in-depth reviews of this ancient field and its modern-day advances, Biocatalysis and Agricultural Biotechnology is an invaluable lab reference for teachers, graduate students, and industrial scientists conducting research in the biosciences.

Biocatalysis and Biomolecular Engineering

Author: Ching T. Hou,Jei-Fu Shaw

Publisher: John Wiley & Sons

ISBN: 9780470920831

Category: Science

Page: 490

View: 4083

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An expert overview of new technologies guiding the construction of a sustainable society This compendium of important insights from sixty distinguished international scholars looks at the significant advances in progressive environmental technology—especially the molecular engineering used on plants, animals, and microorganisms—as the game changer in the high-stakes race to reverse earth-damaging practices. Biocatalysis and Biomolecular Engineering covers subject matter on the latest developments in eco-friendly and energy-saving manufacturing processes with the emphasis on agricultural technology and bio-based products. Focusing its study on remedies that show promise in curing food and energy ills, this book examines groundbreaking work in various fields, such as nutraceuticals, genetic engineering of agricultural products, and bioenergy. Biocatalysis and Biomolecular Engineering: Can be used as a reference by teachers, graduate students, and industrial scientists who conduct research in bioscience and biotechnology Serves as the first book to bring together fundamentals and leading-edge technologies for the development of bio-based industrial products through biocatalysis; for example, it discusses the preparation of biofunctional micro- and nanoparticles Contains chapters by international experts from academia, industry, and government research institutes Biocatalysis and Biomolecular Engineering builds a cohesive, well thought out case for nurturing new discoveries in eco-technology by inviting critical discussion on devising viable solutions to sustaining the future wellness of humankind.

Marine Enzymes for Biocatalysis

Sources, Biocatalytic Characteristics and Bioprocesses of Marine Enzymes

Author: Antonio Trincone

Publisher: Elsevier

ISBN: 1908818352

Category: Science

Page: 576

View: 1552

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Marine bioprospecting is a highly topical subject - in both applied and basic research - but, as yet, the marine ecosystem is a relatively unexplored source of natural bioactive substances with potential therapeutic activity. This book addresses the use of marine enzymes in biocatalysis through a series of chapters from leading scientists within academic and industrial fields. Biocatalytic processes can take advantage of the habitat-related properties of marine enzymes, such as salt tolerance, hyperthermostability, barophilicity, cold adaptivity, and so on, whilst also taking into consideration substrate specificity and affinity. These evolved properties are linked to the metabolic functions of the enzymes and to the ecological aspects of the natural source. New properties can also be discovered at the molecular level of catalysis, particularly concerning the stereochemical characteristics of products. Marine enzymes for biocatalysis initially examines the nature and level of interest in marine biological diversity, and outlines the fundamentals of biocatalysis. It goes on to detail sources of marine enzymes, and to analyse examples from both chemical and stereochemical viewpoints of catalysis, including microbial enzymes and animal or plant sources. The book goes on to explore the future potential of marine bioprospecting in biocatalysis. Compiles studies from leading scientists in a direct and accessible format. Includes practical descriptions of results, adding further details not often covered in formal articles Takes a molecular view which fully explains the enzymatic aspects of reactions, particularly regarding biocatalytic characteristics and descriptions of bioprocesses Selects examples of chemical and stereochemical aspects of enzymatic action with respect to known terrestrial counterparts